Specific plasma membrane receptors for reovirus on rat pituitary cells in culture.

نویسندگان

  • E Maratos-Flier
  • C R Kahn
  • D R Spriggs
  • B N Fields
چکیده

Specific cellular and host tropism is a characteristic property of many viruses mediated by the interaction of viral attachment proteins with components of the plasma membrane of the cell. We have studied the binding of virus to cells quantitatively by using type 3 reovirus labeled with 125I and GH4C1 pituitary cells in culture. Binding was rapid at both 4 degrees and 15 degrees C and was stable over a 9-h period. Unlabeled virus inhibited binding of the labeled virus in a dose-dependent manner. Scatchard analysis revealed 4,200 viral binding sites/cell with an apparent affinity of 1.2 X 10(-11) M. Also, binding of type 3 reovirus was inhibited by antibodies directed against the viral hemagglutinin and partially inhibited by type 2 reovirus, but was unaffected by type 1 reovirus or a variety of other ligands that bind to receptors on GH4C1 cells. These data indicate that reovirus binds to a high affinity, specific receptor on target cells, which may control its tropism and ultimate disease expression.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

The effect of aquatic activity and alogenic bone marrow derived mesenchymal stem cells fortified with Platelet-Rich Plasma in treatment of Achilles tendon in rat

The aim of this study was to the effect of aquatic activity and alogenic bone marrow derived mesenchymal stem cells fortified with Platelet-Rich Plasma in treatment of Achilles tendon in rat.  74 Sprague-Dawley rats were selected and tendon injury was formed in 69 of them. Subsequently, these rats were randomly divided into 8 groups and 5 rats which were without any injuries were chosen as the ...

متن کامل

Lectin Histochemical Study of Vasculogenesis During Rat Pituitary Morphogenesis

Objective(s) The aim of this study was to investigate glycoconjugates distribution patterns as well as their changes during the course of pituitary portal vasculogenesis and angiogenesis. Materials and Methods Formalin fixed paraffin sections of 10 to 20 days of Sprague Dawly rat fetuses were processed for histochemical studies using four different horseradish peroxidase (HRP) conjugated lec...

متن کامل

Human Mesenchymal Stem Cells and Their, Clinical Aapplication

There are two main categories for stem cells a cording to their origin: Embryonic Stem Cells and Adult Stem Cell. Mesenchymal stem cell, supporting hematopoetic stem cells in bone marrow, can regenerate tissues such as bone, cartilage, muscle, tendon and fatty tissue. These cells were recognized for the first time by Friedenstein and Petrokova who could isolate theme from rat bone marrow.Mesenc...

متن کامل

Pectic Acid Effects on Prolactin Secretion in GH3/B6 Rat Pituitary Cell Line

Background: Pectic acid extracted from plants increases the secretion of prolactin (PRL) when injected intravenously into ewes or fed to rats. Fragments of ewe hypophysis and lactating rabbit mammary gland incubated in vitro in the presence of pectic acid secreted more PRL and caseins compared to the controls. However, it is not known whether pectic acid directly stimulates PRL secretion in pi...

متن کامل

Evidence for Histidine Residues on Plasma Membrane Phosphatidate Phosphohydrolase from Rat Liver

Objective(s) Phosphatidate phosphohydrolase (PAP) catalyzes the dephosphorylation of phosphatidic acid to yield Pi and  diacylglycerol. Two different forms of PAP in rat hepatocyte have been reported. PAP1 is located in cytosolic and microsomal fractions and participates in the synthesis of triacylglycerols, phosphatidylcholine, and phosphatidylethanolamine, whereas the other form of phosphati...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of clinical investigation

دوره 72 2  شماره 

صفحات  -

تاریخ انتشار 1983